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Dual cycle amplification and dual signal enhancement assisted sensitive SERS assay of MicroRNA.
- Source :
-
Analytical Biochemistry . Jan2019, Vol. 564, p16-20. 5p. - Publication Year :
- 2019
-
Abstract
- Abstract A sensitive surface-enhanced Raman scattering (SERS) approach has been developed for detection of microRNA (miRNA) based on target-triggered dual signal amplification including strand displancement amplification (SDA) and hybridization chain reaction (HCR). With the assistant of polymerase and nicking endonuclease (NEase), target miRNA combines with the single stranded template DNA to generate a great amount of trigger DNA which can induce HCR. Coupled the dual cycle amplification of SDA and HCR with the dual enhancement of gold nanoparticles (AuNPs), a low detection limit of 0.5 fM for miRNA is obtained using the proposed strategy. With high sensitivity, universality, rapid analysis, and high selectivity, this method has a great potential for detecting biomolecules with trace amounts in bioanalysis and clinical biomedicine. Highlights • A novel method of dual cycle amplification and dual signal enhancement assisted SERS detection for miRNA is developed. • This method allows the transduction of miRNA detection into DNA-based signal amplification. • This assay has the advantages of wide dynamic range, high sensitivity and selectivity. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SERS spectroscopy
*MICRORNA
*POLYMERASES
*ENDONUCLEASES
*GOLD nanoparticles
Subjects
Details
- Language :
- English
- ISSN :
- 00032697
- Volume :
- 564
- Database :
- Academic Search Index
- Journal :
- Analytical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 133012644
- Full Text :
- https://doi.org/10.1016/j.ab.2018.10.004