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Development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts.
- Source :
-
Food & Agricultural Immunology . Dec2018, Vol. 29 Issue 1, p14-26. 13p. - Publication Year :
- 2018
-
Abstract
- The presence of pesticide residues in crops is a significant public concern. Due to the large number of samples and pesticides, a reliable, simple, cost-effective, and high-throughput analytic method is required. In this study, we developed a gold nanoparticle (GNP) immunochromatographic assay (ICA) for the rapid detection of 6-benzylaminopurine (6-BA) residues in bean sprouts. A monoclonal antibody against 6-BA, having a half-maximum inhibition concentration of 2.25 ng/mL, was labeled with GNPs and fixed onto a glass fiber membrane to create a conjugate pad. Coating antigen and goat anti-mouse antibody were sprayed onto a nitrocellulose membrane to form the test line and control line, respectively. The visual limit of detection and cutoff limit of ICA were 10 and 80 ng/g in bean sprouts, respectively. The results of ICA were obtained within 10 min with the naked eye. Therefore, ICA is a rapid tool for the on-site screening and detection of 6-BA in bean sprouts. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 29
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 133102959
- Full Text :
- https://doi.org/10.1080/09540105.2017.1354359