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Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip.
- Source :
-
Food & Agricultural Immunology . Dec2018, Vol. 29 Issue 1, p358-368. 11p. - Publication Year :
- 2018
-
Abstract
- In this paper, we describe the development of a rapid, simple, specific, and sensitive immunochromatographic strip test based on an antibody-antigen reaction for detection of tulathromycin (TULA) in samples of 0.01 M PBS pH 7.4, pure milk and honey. The monoclonal antibodies specifically recognized the corresponding antigens produced in our laboratory. Under optimized conditions, the cut-off limits of the test strips for TULA in 0.01 M PBS pH 7.4 was found to be 2.5 ng/mL. Meanwhile, with simple preparation for detection in pure milk and honey samples, the cut-off limits for TULA were found to be 5 and 10 ng/mL, respectively. Each test can be evaluated in 5 min. In summary, this lateral-flow device provides a sensitive, effective, specific, and rapid method for detection of TULA residues in food matrix. [ABSTRACT FROM AUTHOR]
- Subjects :
- *TULATHROMYCIN
*MONOCLONAL antibodies
*HONEY
*ANTIGEN-antibody reactions
*MILK
Subjects
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 29
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 133102986
- Full Text :
- https://doi.org/10.1080/09540105.2017.1376040