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Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine.
- Source :
-
Food & Agricultural Immunology . Dec2018, Vol. 29 Issue 1, p434-444. 11p. - Publication Year :
- 2018
-
Abstract
- Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine. [ABSTRACT FROM AUTHOR]
- Subjects :
- *OCHRATOXINS
*RED wines
*MYCOTOXINS
*MONOCLONAL antibodies
*PHOSPHATES
Subjects
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 29
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 133102992
- Full Text :
- https://doi.org/10.1080/09540105.2017.1401043