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Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody.
- Source :
-
Food & Agricultural Immunology . Dec2018, Vol. 29 Issue 1, p930-940. 11p. - Publication Year :
- 2018
-
Abstract
- The problem of waste oil is a food safety issue that requires urgent resolution. In this study, a novel hapten was designed for the rapid detection of capsaicinoids, a marker of waste oil, and a monoclonal antibody (MAb) 5D7 was generated that is specific for three major capsaicinoids: capsaicin (CPC), dihydrocapsaicin (DCPC), and N-vanillylnonanamide (N-V). The half maximal inhibitory concentrations of MAb 5D7 for CPC, DCPC, and N-V were 1.12, 0.8, and 0.87 ng/mL, respectively. An immunochromatographic strip test based on MAb 5D7 was established for these three capsaicinoids, with visual inspection limits of <1.0 ng/mL, which satisfies the requirement for the rapid on-site inspection of waste oil. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CAPSAICINOIDS
*MONOCLONAL antibodies
*PETROLEUM waste
*FOOD safety
*IMMUNOASSAY
Subjects
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 29
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 133103031
- Full Text :
- https://doi.org/10.1080/09540105.2018.1490394