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Enhanced doxorubicin production by Streptomyces peucetius using a combination of classical strain mutation and medium optimization.
- Source :
-
Preparative Biochemistry & Biotechnology . 2018, Vol. 48 Issue 6, p514-521. 8p. 1 Color Photograph, 1 Diagram, 1 Chart, 5 Graphs. - Publication Year :
- 2018
-
Abstract
- Doxorubicin (DXR), which is produced by Streptomyces peucetius, is an important anthracycline-type antibiotic used for the treatment of various cancers. However, due to the low DXR productivity of wild-type S. peucetius, it is difficult to produce DXR by one-step fermentation. In this study, a DXR-resistance screening method was developed to screen for DXR high-producing mutants. Then, S. peucetius SIPI-11 was treated several times with UV and ARTP (atmospheric and room temperature plasma) to induce mutations. Treated strains were screened by spreading on a DXR-containing plate, isolating a mutant (S. peucetius 33-24) with enhanced DXR yield (570 mg/L vs. 119 mg/L for the original strain). The components of the fermentation medium, including the carbon and nitrogen sources, were optimized to further enhance DXR yield (to 850 mg/L). The pH of the fermentation medium and culture temperature were also optimized for effective DXR production. Finally, DXR production by S. peucetius 33-24 was investigated in flask culture and a fermenter. The yield of DXR was as high as 1100 mg/L in a 5-L fermenter, which is the highest DXR productivity reported thus far, suggesting that S. peucetius 33-24 has the potential to produce DXR by direct fermentation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DOXORUBICIN
*STREPTOMYCES
*GENETIC mutation
*ANTHRACYCLINES
*FERMENTATION
Subjects
Details
- Language :
- English
- ISSN :
- 10826068
- Volume :
- 48
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Preparative Biochemistry & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 133175375
- Full Text :
- https://doi.org/10.1080/10826068.2018.1466156