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Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510
- Source :
-
Carbohydrate Research . Jun2004, Vol. 339 Issue 8, p1475-1481. 7p. - Publication Year :
- 2004
-
Abstract
- Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed. [Copyright &y& Elsevier]
- Subjects :
- *OLIGOSACCHARIDES
*ALGINATES
*HYDROLYSIS
*SPECTRUM analysis
Subjects
Details
- Language :
- English
- ISSN :
- 00086215
- Volume :
- 339
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Carbohydrate Research
- Publication Type :
- Academic Journal
- Accession number :
- 13333604
- Full Text :
- https://doi.org/10.1016/j.carres.2004.03.010