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Synthetic Antigens Composed Exclusively of L-or D-Amino acids I. EFFECT OF OPTICAL CONFIGURATION ON THE IMMUNOGENICITY OF SYNTHETIC POLYPEPTIDES IN MICE.
- Source :
-
Immunology . Jul67, Vol. 13 Issue 1, p29-38. 10p. - Publication Year :
- 1967
-
Abstract
- Two random linear co-polymers composed exclusively of D-amino acids were found to elicit a primary response in mice; the sera were assayed by the indirect precipitin reaction. 247, p (D-Tyr, D-Glu, D-Ala) was studied intensively and compared to a similar polypeptide, 253, p (L-Tyr, L-Glu, L-Ala), of op- posite optical configuration. The primary response to the D-isomer in adjuvant is highly dose-dependent, showing a sharp maximum around 1 μg/mouse. while that to the L-isomer in adjuvant is largely dose-independent. At low doses the D-isomer is as immunogenic as the L-isomer. Re-injection of the D-isomer led to no increase in titre; re-injection of the L-isomer gave a typical secondary response. The antibodies formed to both isomers were highly stereospecific. The D-isomer was 50–1000 times as efficient at inducing paralysis as the L-isomer, and the paralysis was specific. The differences between the D- and L-isomers are thought to be explained by the much greater capacity of the D-isomer in inducing immunological paralysis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13336808