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MicroRNA-126 attenuates cell apoptosis by targeting TRAF7 in acute myeloid leukemia cells.
- Source :
-
Biochemistry & Cell Biology . 2018, Vol. 96 Issue 6, p840-846. 7p. - Publication Year :
- 2018
-
Abstract
- Acute myeloid leukemia (AML) has a 5-year survival rate of only about 30%–40% due to the self-renewal and differentiation ability of leukemia stem-like cells (LSCs). To address the potential for novel therapeutic targets in LSCs, we investigated the roles of miRNA-126 and tumor necrosis factor receptor-associated factor 7 (TRAF7) in AML. We used qRT-PCR and Western blot to investigate the expression levels of miRNA-126 and TRAF7 in AML cell lines. Then, we uncovered the effect of miRNA-126 on AML cell proliferation and apoptosis by MTT assay and flow cytometric analysis, respectively. Furthermore, dual-luciferase assay and Western blot were used to determine the target of miRNA-126 in AML and the potential mechanism by which cell apoptosis is suppressed by miRNA-126. We found that miRNA-126 was highly expressed in all of the AML cell lines, and that inhibition of miRNA-126 significantly induced cell death through apoptosis. The suppression of apoptosis in AML with high expression of miRNA-126 was caused by down-regulating TRAF7, which blocked the c-FLIP pathway. The role of miRNA-126 in AML makes it a potential therapeutic target to improve clinical outcomes for patients with AML. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08298211
- Volume :
- 96
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Biochemistry & Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 133393492
- Full Text :
- https://doi.org/10.1139/bcb-2018-0017