FS-7 inhibits MGC-803 cells growth in vitro and in vivo via down-regulating glycolysis.

Graphical abstract Highlights • FS-7 had potent anticancer activity in human gastric carcinoma MGC-803 cell line. • FS-7 had potent anticancer activity in vivo/vitro and was superior to 5-Fu. • FS-7 down-regulated glycolysis-related protein HIF-1α, HK-II and PFKP. Abstract In this study, we investigated the anticancer effects of FS-7, a flavonoid salicylate derivative, in human gastric carcinoma MGC-803 cell line and studied its preliminary anticancer effects. FS-7 displayed greater in vitro cytotoxicity against MGC-803 cell line compared with 5-Fu and had a certain extent of selectivity to cancer cells. The flow cytometry analysis revealed that FS-7 induced apoptosis MGC-803 cells and mainly caused cells arrest in the G2/M phase in a concentration-dependent manner. Additionally, FS-7 inhibited the colony formation and cell migration in a concentration-dependent manner. Notably, FS-7 noticeably down-regulated glycolysis-related protein HIF-1α, HK-II and PFKP expression in a concentration-dependent manner, possibly causing glycolysis inhibition. Importantly, compared with 5-Fu, FS-7 showed better anticancer activity in the MGC-803 xenograft murine tumor models. Collectively, the present study provided a promising anticancer drug candidate for gastric cancer therapy. [ABSTRACT FROM AUTHOR]