Back to Search Start Over

Silica-Based Nanoparticles for Protein Encapsulation and Delivery.

Authors :
Begarani, Filippo
Cassano, Domenico
Margheritis, Eleonora
Marotta, Roberto
Cardarelli, Francesco
Voliani, Valerio
Source :
Nanomaterials (2079-4991). Nov2018, Vol. 8 Issue 11, p886. 1p.
Publication Year :
2018

Abstract

Although conceptually obvious, the effective delivery of proteins in therapeutic applications is far from being a routine practice. The major limitation is the conservation of protein physicochemical identity during the transport to the target site. In this regard, nanoparticle-based systems offer new intriguing possibilities, provided that (i) the harsh and denaturating conditions typically used for nanoparticle synthesis are avoided or mitigated; and (ii) nanoparticle biocompatibility and degradation (for protein release) are optimized. Here, we tackle these issues by starting from a nanoparticle architecture already tested for small chemical compounds. In particular, silica-shielded liposomes are produced and loaded with a test protein (i.e., Green Fluorescent Protein) in an aqueous environment. We demonstrate promising results concerning protein encapsulation, protection during intracellular trafficking and final release triggered by nanoparticle degradations in acidic organelles. We believe this proof of principle may open new applications and developments for targeted and efficient protein delivery. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20794991
Volume :
8
Issue :
11
Database :
Academic Search Index
Journal :
Nanomaterials (2079-4991)
Publication Type :
Academic Journal
Accession number :
133520250
Full Text :
https://doi.org/10.3390/nano8110886