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A genome-wide dsRNA library screen for Drosophila genes that regulate the GBP/phospholipase C signaling axis that links inflammation to aging.
- Source :
-
BMC Research Notes . 12/13/2018, Vol. 11 Issue 1, pN.PAG-N.PAG. 1p. 2 Charts, 1 Graph. - Publication Year :
- 2018
-
Abstract
- Objective: Invertebrates are productive models for understanding how inflammation, metabolism and aging are intertwined. We have deployed a dsRNA library screen to search for genes in Drosophila melanogaster—and hence identify human orthologs—that encode participants in a G-protein coupled, Ca2+-signaling pathway that regulates inflammation, metabolism and lifespan. Results: We analyzed receptor-dependent, phospholipase C/Ca2+ signaling responses to the growth-blocking peptide (GBP) cytokine in Drosophila S3 cells plated in 384-well plates containing dsRNAs that target approximately 14,000 Drosophila genes. We used Z-scores of < − 3 or > + 3 to define gene hits. Filtering of 'housekeeping' genes from these hits yielded a total of 82 and 61 Drosophila genes that either down-regulate or up-regulate Ca2+-signaling, respectively; representatives from these two groups were validated. Human orthologs of our hits may be modulators of Ca2+ signaling in general, as well as being candidates for acting in molecular pathways that interconnect aging and inflammation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17560500
- Volume :
- 11
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- BMC Research Notes
- Publication Type :
- Academic Journal
- Accession number :
- 133561166
- Full Text :
- https://doi.org/10.1186/s13104-018-3996-z