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Chemometric analysis of porcine, bovine and ovine heparins.
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . Feb2019, Vol. 164, p345-352. 8p. - Publication Year :
- 2019
-
Abstract
- Highlights • 1H-NMR of heparins from different animals show distinct clusters in PCA and k means analysis. • Disaccharides components of heparins from different animals show distinct clusters in PCA and k means. • Tetrasaccharides components of heparins from different animals show distinct clusters in PCA and k means. • Chemometric analysis could help predict the heparin animal source and detect blending. Abstract Heparin is a polysaccharide anticoagulant drug isolated from animal tissues. There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption. The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins. These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site. Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CHEMOMETRICS
*HEPARIN
*DISACCHARIDES
*ANTICOAGULANTS
*POLYSACCHARIDES
Subjects
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 164
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 133580281
- Full Text :
- https://doi.org/10.1016/j.jpba.2018.10.052