Nitrate transporter NPF7.3/NRT1.5 plays an essential role in regulating phosphate deficiency responses in Arabidopsis.

Abstract AtNPF7.3/AtNRT1.5, which is a nitrate transporter that drives root-to-shoot transport of NO 3 −, is also involved in modulating the response to K+ deprivation in Arabidopsis by affecting root development and K+ transport. However, whether NPF7.3/NRT1.5 functions in regulating plant responses to deficiencies of other nutrients remains unknown. In this study, we found that the expression of AtNPF7.3/AtNRT1.5 was predominant in the roots and was substantially induced by phosphate (Pi) starvation. The atnrt1.5 mutants displayed conspicuously longer primary roots along with a significantly reduced lateral root density under Pi-deficient conditions than did the wild-type plants, and these morphological differences in the roots were eliminated to a certain extent by the ethylene synthesis antagonist Co2+. Further analyses revealed that the expression of important Pi starvation-induced genes, which are directly involved in Pi transport, mobilization and distribution, were significantly higher in the atnrt1.5 mutants than that in the wild-type plants under Pi-starvation conditions; therefore, the atnrt1.5 mutants retained higher tissue Pi concentrations. Taken together, our results suggest that NPF7.3/NRT1.5 is an important component in the regulation of phosphate deficiency responses in Arabidopsis. Highlights • Expression of NRT1.5 was strongly induced by Pi-starvation. • Nrt1.5 mutants displayed altered root architecture under Pi-deficiency. • Nrt1.5 mutants might lose the ethylene responsiveness under Pi-deficiency. • The mutation of NRT1.5 enhanced the expression of Pi starvation-induced genes. • The mutation of NRT1.5 increased the tissue Pi content under Pi-deficiency. [ABSTRACT FROM AUTHOR]