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Simultaneous fermentation of biomass-derived sugars to ethanol by a co-culture of an engineered Escherichia coli and Saccharomyces cerevisiae.
- Source :
-
Bioresource Technology . Feb2019, Vol. 273, p269-276. 8p. - Publication Year :
- 2019
-
Abstract
- Graphical abstract Highlights • Glucose and xylose were co-fermented by a co-culture of engineered E. coli and yeast. • Co-fermentation of sugars from sugar cane bagasse slurry at 20% solids was achieved. • Xylose fermentation rate of E. coli in media with glucose was 1.58 ± 0.21 g.L−1.h−1. Abstract Microorganisms ferment xylose at high rate only when glucose concentration in the medium falls below a critical level. Since the specific productivity of product is highest during exponential to early stationary phase of growth, a glucose utilization negative ethanologenic E. coli (strain LW419a) was constructed for high rate of xylose fermentation in combination with Turbo yeast. This co-culture fermented all the released sugars in an acid/enzyme-treated sugar cane bagasse slurry (10% solids) to an ethanol titer of 24.9 ± 0.8 g.L−1 (70% of the theoretical yield) in <30 h. Ethanol titer increased to 48.6 ± 1.04 g.L−1 (yield, 0.45 g.g−1 sugars) at a solids content of 20% and the highest rate of xylose consumption was 1.58 ± 0.21 g.L−1.h−1. This study demonstrates the potential of a co-culture of strain LW419a and yeast to rapidly ferment all the sugars in pretreated biomass slurries to ethanol at their respective highest rates. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 273
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 133622427
- Full Text :
- https://doi.org/10.1016/j.biortech.2018.11.016