CircPUM1 promotes the malignant behavior of lung adenocarcinoma by regulating miR-326.

Abstract CircRNAs are reported to be implicated in the development of lung cancer. This study focused on assessing the expression, functions and molecular mechanism of circPUM1 in lung adenocarcinoma. Here, it showed that circPUM1 is significantly upregulated in both lung adenocarcinoma cell lines and tissues. Furthermore, silencing of circPUM1 impaired the proliferation, migration and invasion ability, and increased apoptosis in A549 cells. Nevertheless, overexpression of circPUM1 in SPC-A1 cells has the opposite effect. Silencing of circPUM1 inhibits the tumorigenesis in nude mice. Mechanistically, circPUM1 could sponge miR-326 and promote the expression of its downstream proteins Cyclin D1 and Bcl-2. In summary, this present study revealed that circPUM1 functions as an oncogene to promote the tumorigenesis of lung adenocarcinoma through circPUM1/miR-326/Cyclin D1 and Bcl-2 axis. This indicates that circPUM1 may act as a potential therapeutic target for lung adenocarcinoma. Highlights • Upregulation of circPUM1 in lung adenocarcinoma cell lines and tissues. • Silencing of circPUM1 inhibits the proliferation, migration, invasion and inhibited apoptosis in vitro, and vice versa. • CircPUM1 sponges miR-326 to upregulate Cyclin D1 and Bcl-2 expression to promote tumorigenesis. [ABSTRACT FROM AUTHOR]