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Monocyte-T-cell interactions in pokeweed mitogen-activated cultures.
- Source :
-
Immunology . Nov90, Vol. 71 Issue 3, p397-403. 7p. - Publication Year :
- 1990
-
Abstract
- Monocyte-T-cell interactions were studied in pokeweed mitogen (PWM)-activated cell cultures. We addressed the question of monocyte changes in PWM-stimulated cultures of T cells and monocytes and found, by flow cytometric analysis, that PWM activation led to a loss of cells with monocyte or macrophage phenotype (CD14, HLA-DR, HLA-DQ) within 48 hr of culture in the presence of T cells (CD4+ T cells), but not in cultures of pure monocytes. Chemiluminescence measurements revealed that phagocytic stimulation of monocytic superoxide release was impaired in PWM-stimulated cultures of monocytes plus T cells, but not in PWM-stimulated cultures of pure monocytes. Furthermore, PWM induced the secretion of interferon-gamma (IFN-γ) in primary cultures oft cells supplemented with 20% of monocytes, whereas in subsequent secondary cultures of these cells PWM induced IFN-γ only when monocytes were added. We conclude from these flow cytometric and functional analyses that monocytes are efficiently eliminated from PWM-activated T-cell/monocyte cultures by CD4+ T lymphocytes. [ABSTRACT FROM AUTHOR]
- Subjects :
- *T cells
*POKEWEED mitogens
*PLANT lectins
*MITOGENS
*MONOCYTES
*IMMUNOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 71
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13384914