抑制 GATA5 蛋白可否促进 HELA 细胞中癌干细胞因子 sox2、 c-myc 及 CD44 的表达.

BACKGROUND: GATA5, as a tumor suppressor gene, plays a role in inhibiting the development of cancer. GATA5 can be methylated in gastrointestinal tumors and then lose its function. GATA5 can be also methylated during hepatocarcinogenesis, and then cannot promote the differentiation of stem cells and maintain the normal function. Cervical cancer HELA cells express GATA5 protein, in which whether GATA5 plays a tumor suppressor function is still unknown. OBJECTIVE: To analyze the expression of sox2, c-myc and CD44 related to cancer stem cell formation after suppressing the expression of GATA5 in cervical cancer HELA cells. METHODS: Transfection of CMV-siRNA-gata5 further inhibited the expression of GATA5 in cervical cancer HELA cells. The expression of c-myc mRNA and sox2 mRNA were then detected by RT-PCR. The proliferation of HELA cells was detected by MTT. The colony formation and soft agar monoclonal formation of HELA cells were observed. The expression of GATA5, c-myc and sox2 was detected by western blot. The expression and location of CD44 was detected by laser confocal microscope. RESULTS AND CONCLUSION: After suppressing the expression of GATA5 in cervical cancer HELA cells by CMV-siRNA-gata5, the proliferation, cell monoclonal formation and colony formation of HELA cells were enhanced. The expression of c-myc, sox2 and CD44 was also strengthened. These findings indicate that the malignant activity of HELA cells is increased by inhibiting the GATA5 protein expression. The mechanism may be that inhibition of GATA5 promotes the expression of cancer stem cell factors sox2, c-myc, and CD44. [ABSTRACT FROM AUTHOR]