脂肪间充质干细胞复合多孔壳聚糖微球修复大鼠肝损伤.

BACKGROUND: With the development of stem cell therapy and tissue engineering, adipose-derived mesenchymal stem cells combined with biocompatible scaffolds have been introduced to constructing liver tissue engineering micro-units as a new strategy for the treatment of liver diseases. OBJECTIVE: To investigate the repairing ability of adipose-derived mesenchymal stem cells combined with porous chitosan microspheres on acute liver injury. METHODS: Adipose-derived mesenchymal stem cells from neonatal Sprague-Dawley rats and porous chitosan microspheres were co-cultured in vitro to observe the proliferation of adipose-derived mesenchymal stem cells on the microspheres on the 1st, 3rd and 5th days. Thirty-six Sprague-Dawley rats (provided by Beijing Vital River Laboratory Animal Technology Co., Ltd. in China) were randomly divided into three groups (n=12 per group): control group was intraperitoneally injected with normal saline; acute liver injury group was intraperitoneally administered with 10% carbon tetrachloride-olive oil; and cell-microsphere group was intraperitoneally given 10% carbon tetrachloride-olive oil and then adipose-derived mesenchymal stem cells-porous chitosan microspheres were implanted into the liver surface on the 1st day after injection. Gross, serological and histological evaluations were performed on the 1st, 2nd, 3rd and 7th days. RESULTS AND CONCLUSION: Adipose-derived mesenchymal stem cells-porous chitosan microspheres were stained with live/dead staining on the 1st, 3rd and 5th days. The cells grew normally on the microspheres as the number of dead cells was much less than that of living cells, and the cells proliferated continuously over time. The gross score, liver function score and histological score of the cell-microsphere group were higher than those of the acute liver injury group on the 1st, 2nd, 3rd and 7th days. There was no significant difference between the cell-microsphere group and control group since the 3rd day. All these experimental findings indicate that adipose-derived mesenchymal stem cells-porous chitosan microspheres implanted onto the liver surface can accelerate the repair of liver injury. [ABSTRACT FROM AUTHOR]