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Detección del virus del dengue en larvas y pupas de Aedes aegypti recolectadas en áreas rurales del municipio de Anapoima, Cundinamarca, Colombia.

Authors :
Lucía Velandia-Romero, Myriam
Alberto Olano, Víctor
Coronel-Ruiz, Carolina
Cabezas, Laura
Angélica Calderón-Peláez, María
Eduardo Castellanos, Jaime
Inés Matiz, María
Source :
Revista Biomedica. 2017, Issue Suplemento 2, p1-1. 1p.
Publication Year :
2017

Abstract

Introduction: There is a high incidence and prevalence of dengue in the department of Cundinamarca, and recently Aedes aegypti, the main vector of dengue virus (DENV), was detected in some of its rural areas.. Objective: To evaluate viral transovarial transmission in larvae and pupae collected in rural areas of the municipality of Anapoima, Cundinamarca. Materials and methods: Live larvae and pupae were collected from 53 homes and later they were taken to the laboratory in Anapoima, where they were classified, pooled and frozen. In Bogotá, they were homogenized, RNA was extracted with Trizol™, and RT-PCR and conventional PCR were performed. The amplified products were analyzed on 2% agarose gels. Results: In 54.7% of the houses we found A. aegypti in immature stages, and DENV-1 was the most frequent serotype. However, the simultaneous presence of DENV 1 and 2, DENV 1 and 3, DENV 1 and 4, and DENV 1, 2 and 3 serotypes was detected in some pools. Conclusion: The results confirmed the natural vertical transmission of the virus in the rural area under study. These findings confirmed the vector capacity of A. aegypti, and partly explains the persistence of the virus in the region and the possibility of transmission by the vector during adulthood without having ingested infected blood. This situation increases the risk of DENV infection in Colombia and the need for prevention and control programs in all areas where the mosquito is present. [ABSTRACT FROM AUTHOR]

Details

Language :
Spanish
ISSN :
0188493X
Issue :
Suplemento 2
Database :
Academic Search Index
Journal :
Revista Biomedica
Publication Type :
Academic Journal
Accession number :
134045181
Full Text :
https://doi.org/10.7705/biomedica.v34i2.3584