The Effect of ζ‐Potential and Hydrodynamic Size on Nanoparticle Interactions in Hydrogels.

The ζ‐potential and hydrodynamic size (dh) of nanoparticles (NPs) are systematically controlled by capping gold NPs (AuNPs) with polymers having different charges and treating them in NaCl solutions of diverse concentrations. Interactions between AuNPs in hydrogel are caused by chemical reactions induced by 1,4‐dithiothreitol. The effect of ζ‐potential is clear, as negatively charged AuNPs can be aggregated in neutral agarose gel, but the amount of aggregation is significantly affected by the magnitude of the negative surface charge on the AuNPs. However, all positively charged AuNPs show negligible aggregation in agarose gel with slightly negative polarity. The effect of dh on AuNP aggregation is different from that of ζ‐potential. Although AuNPs with small dh generally show more aggregation than those with large dh, the amount of AuNP capping layer is critical. Thus, the amount of polymer present on NP surface needs to be considered to investigate the effect of dh on AuNP aggregation. Through extended Derjaguin, Landau, Verwey, Overbeek (XDLVO) theory, it is shown that the charges of the AuNPs and the hydrogel, as well as the dh of the NPs, are related to electrostatic repulsion and steric hindrance, which affect AuNP aggregation in hydrogel. Chemical interaction of nanoparticles in hydrogel is significantly affected by their ζ‐potential and hydrodynamic size. The charges of nanoparticles and the hydrogel and the hydrodynamic size of the nanoparticles affect the interaction of nanoparticles in hydrogel via electrostatic repulsion and steric hindrance occurring between them. [ABSTRACT FROM AUTHOR]