Vertical distribution and assemblages of microbial communities and their potential effects on sulfur metabolism in a black-odor urban river.

Abstract Black-odor phenomenon in highly urbanized river is increasingly recognized as a global ecological risk. Biotransformation associated with sulfur cycle is a major contributor to the blank-odor phenomenon. The vertical geochemical gradient in black-odor rivers is likely to alter microbial community assemblages and functions in the sulfur cycle. However, the interactions between geochemical gradients and microbial communities, as well as the changes in the process of sulfur biotransformation under different environmental conditions remain largely unknown. The vertical community assembly patterns and the impacts of microbial communities and genes on the biotransformation in the sulfur cycle were revealed in our study for the first time in a typical urban black-odor river, Jinchuan River, in China. Vertical beta-diversity patterns of microbial communities mainly resulted from species replacement that was largely driven by spatial turnover (β SIM = 0.43) but also influenced by nestedness (β NES = 0.08). MiSeq sequencing and GeoChip 5.0 microarray chip approaches were applied and identify 41 bacterial genera, 8 archaeal genera, and 26 genes involved in the sulfur cycle in Jinchuan River. The vertical beta-diversity patterns of microbial profile mainly resulted from species replacement. Those sulfur-related bacterial and archaeal genera, accounting for 23.15% and 42.65% of the total bacteria and archaea respectively in analysed samples, were mainly responsible for sulfur reduction. According to redundancy analysis, oxidation-reduction potential (r = −0.8662, P < 0.05), S2− concentration (r = −0.6288, P < 0.05), and total nitrogen concentration (r = −0.6782, P < 0.05) were identified as factors that significantly affect sulfur-related microbial communities. The highest reaction potential was detected in the dissimilated sulfate reduction action and experienced an increase with depth increasing in the river system. The results indicated that the sulfur biotransformation in a deeper layer in river sediment could make more contribution to the black-odor phenomenon in urban rivers. Graphical abstract Image 1 Highlights • Vertical structured microbial communities in an urban river were detected. • Functions of microbial community on sulfur biotransformation were detected. • Remarkable micro-scale differentiations were found in different depths of rivers. • Vertical microbial beta-diversity patterns were largely driven by spatial turnover. • Sulfur biotransformation in deeper sediment contributes more to river pollution. [ABSTRACT FROM AUTHOR]