辣椒素抑制成纤维细胞增殖的作用及其分子机制.

BACKGROUND: Capsaicin has been reported to inhibit fibrosis and the proliferation and collagen synthesis of hypertrophic fibroblasts. However, the underlying mechanism is unclear. OBJECTIVE: To investigate the effect of capsaicin on inhibiting the proliferation of fibroblasts and the corresponding molecular mechanism. METHODS: Neonatal Sprague-Dawley rat epidermal fibroblasts were cultured in vitro, and were treated with capsaicin at different concentrations (0.5, 1, 2, 4, 8 mg/L) for 24 hours. The cells without treatment of capsaicin were included in the negative control group. Morphological changes of the epidermal fibroblasts were observed under phase-contrast microscope. The cell viability and IC50 value were detected by MTT assay. The cell apoptosis was detected by Hoechst 33258 staining and AnnexinV-FITC/PI staining. The cell cycle distribution was determined by flow cytometry. The protein expression levels of FoxO1, p-FoxO1, Akt and p-Akt were detected by western blot assay. RESULTS AND CONCLUSION: With capsaicin concentration increasing, cell survival rate decreased gradually in a concentration-dependent manner, and IC50 value was 3.55 mg/L. Compared with the negative control group, after 24 hours, capsaicin treated fibroblasts stopped in G1 phase and the apoptosis rate increased. Meanwhile, capsaicin could decrease the expression of P-Akt and P-FoxO1. Therefore, capsaicin can inhibit the proliferation of fibroblasts, promote cell apoptosis, and present a concentration-dependent effect. The Akt-FoxO1 signaling pathway may be involved in the inhibition of capsaicin on fibroblast proliferation. [ABSTRACT FROM AUTHOR]