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Covalent modification of β-lactoglobulin by (−)-epigallocatechin-3-gallate results in a novel antioxidant molecule.
- Source :
-
International Journal of Biological Macromolecules . Apr2019, Vol. 126, p1186-1191. 6p. - Publication Year :
- 2019
-
Abstract
- Abstract β-lactoglobulin (β-lg), the predominant protein in bovine whey, was chemically modified by (−)-epigallocatechin-3-gallate (EGCG) to develop a biomacromolecule with antioxidant activity. The EGCG-modified β-lg was characterized by SDS-PAGE, MALDI-TOF MS and intrinsic fluorescence spectroscopy. The antioxidant properties of EGCG-modified β-lg was assessed using DPPH radical scavenging activity, iron chelating ability, and inhibition of Cu2+-induced LDL oxidation. SDS-PAGE and MALDI-TOF MS results indicated the dimerization of β-lg after EGCG modification. Intrinsic fluorescence spectra suggested that EGCG modification caused an alteration in the conformational structure of β-lg. The results demonstrated that EGCG-modified β-lg possessed great antioxidant potential in terms of scavenging DPPH radical and chelating ferrous ion. Furthermore, the EGCG-modified β-lg showed a protective effect against LDL peroxidation. The results indicate that EGCG-modified β-lg could provide significant health benefits as an antioxidant. Highlights • Covalent modification by EGCG caused the formation of β-lg dimer. • EGCG modification altered β-lg conformation making it more hydrophilic. • EGCG-modified β-lg is a novel biomacromolecule with antioxidant activity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 126
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 134687807
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2019.01.017