A turn-on reactive fluorescent probe for Hg2+ in 100% aqueous solution.

Abstract It is an effective and precise strategy to design the fluorescence probe for Hg2+ based on a new specific reaction. A novel turn-on fluorescent probe, 4-{2-[4-(2-Mercapto-benzoyloxy)-phenyl]-vinyl}-1-methyl-pyridinium[ e ]iodide (MBPVP) for Hg2+ was developed. The mechanism was based on Hg2+-mediated ester hydrolysis to release the fluorophore, 4-[2-(4-Hydroxy-phenyl)-vinyl]-1-methyl-pyridinium[ e ]iodide, which showed outstanding intramolecular charge transfer (ICT) and the yellow-green fluorescence were observed. The turn-on probe showed rapid response time, outstanding selectivity and sensitivity for Hg2+ over other metal ions in 100% PBS buffer (pH = 7.4). The linear range was 2–16 µM and the detection limit was 6.5 nM. Furthermore, MBPVP was used to determine Hg2+ in real samples and in the solid state. Graphical abstract fx1 Highlights • A turn-on fluorescence probe (MBPVP) for Hg2+ based on new specific reaction was developed. • MBPVP showed rapid response time, high selectivity and sensitivity in 100% aqueous solution. • The probe was applied in water samples and in the solid state. [ABSTRACT FROM AUTHOR]