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Surgery-free video-oculography in mouse models: enabling quantitative and short-interval longitudinal assessment of vestibular function.
- Source :
-
Neuroscience Letters . Mar2019, Vol. 696, p212-218. 7p. - Publication Year :
- 2019
-
Abstract
- Highlights • Surgery-free VOR measurement that allows screen for genes and drugs affecting vestibular function has been enabled. • Daily-progression profile of the vestibular impairment caused by IDPN at different dose levels has been obtained. • Mice with homozygous mutations of Lhfpl5 and Cdh23 have been identified based on their reduced VOR-response levels. Abstract Vestibulo-ocular reflex (VOR) responding to acceleration stimuli is originated from the vestibular apparatuses and thus widely used as an in vivo indicator of the vestibular function. We have developed a vestibular function testing (VFT) system that allows to evaluate VOR response with improved efficiency. The previously required surgical procedure has been avoided by using a newly designed animal-immobility setup. The efficacy of our VFT system was demonstrated on the mice with vestibular abnormalities caused by either genetic mutations (Lhfpl5−/− or Cdh23−/−) or applied vestibulotoxicant (3,3′-iminodipropionitrile, IDPN). Daily longitudinal inspection of the VOR response in the IDPN-administered mice gives the first VOR-based daily-progression profile of the vestibular impairment. The capability of VOR in quantifying the severity of toxicant-induced vestibular deficits has been also demonstrated. The acquired VOR-measurement results were validated against the corresponding behavioral-test results. Further validation against immunofluorescence microscopy was applied to the VOR data obtained from the IDPN-administered mice. We conclude that the improved efficiency of our surgery-free VFT system, firstly, enables the characterization of VOR temporal dynamics and quantification of vestibular-impairment severity that may reveal useful information in toxicological and/or pharmaceutical studies; and, secondly, confers our system promising potential to serve as a high-throughput screener for identifying genes and drugs that affect vestibular function. [ABSTRACT FROM AUTHOR]
- Subjects :
- *VESTIBULAR function tests
*VESTIBULO-ocular reflex
*VESTIBULAR apparatus
*MICE
Subjects
Details
- Language :
- English
- ISSN :
- 03043940
- Volume :
- 696
- Database :
- Academic Search Index
- Journal :
- Neuroscience Letters
- Publication Type :
- Academic Journal
- Accession number :
- 134927620
- Full Text :
- https://doi.org/10.1016/j.neulet.2018.12.036