The moss jasmonate ZIM-domain protein PnJAZ1 confers salinity tolerance via crosstalk with the abscisic acid signalling pathway.

Highlights • PnJAZ1 is a stress-induced gene from Antarctic moss and its protein levels are reduced by OPDA treatment. • PnJAZ1 interacts with the AtMYC2 transcription factor and mediates the conserved jasmonate signalling pathway. • The overexpression of PnJAZ1 increases the tolerance of transgenic plants to salt and osmotic stresses and ABA treatment. • PnJAZ1 regulates interactions between the jasmonate and ABA signalling pathways. Abstract Abscisic acid (ABA) and jasmonates (JAs) are the primary plant hormones involved in mediating salt tolerance. In addition, these two plant hormones exert a synergistic effect to inhibit seed germination. However, the molecular mechanism of the interaction between ABA signalling and JA signalling is still not well documented. Here, a moss jasmonate ZIM-domain gene (PnJAZ1), which encodes a nucleus-localized protein with conserved ZIM and Jas domains, was cloned from Pohlia nutans. PnJAZ1 expression was rapidly induced by various abiotic stresses. The PnJAZ1 protein physically interacted with MYC2 and was degraded by exogenous 12-oxo-phytodienoic acid (OPDA) treatment, implying that the JAZ protein-mediated signalling pathway is conserved in plants. Transgenic Arabidopsis and Physcomitrella plants overexpressing PnJAZ1 showed increased tolerance to salt stress and decreased ABA sensitivity during seed germination and early development. The overexpression of PnJAZ1 inhibited the expression of ABA pathway genes related to seed germination and seedling growth. Moreover, the transgenic Arabidopsis lines exhibited enhanced tolerance to auxin (IAA) and glucose, mimicking the phenotypes of abi4 or abi5 mutants. These results suggest that PnJAZ1 acts as a repressor, mediates JA-ABA synergistic crosstalk and enhances plant growth under salt stress. [ABSTRACT FROM AUTHOR]