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Alterations of neuroimmune cell density and pro-inflammatory cytokines in response to thimerosal in prefrontal lobe of male rats.
- Source :
-
Drug & Chemical Toxicology . Mar2019, Vol. 42 Issue 2, p176-186. 11p. - Publication Year :
- 2019
-
Abstract
- Evidence suggests that the effect of heavy metals on neuroimmune cells lead to neurogenic inflammatory responses. In this study, immune cells [mast cells (MCs) and microglia] and pro-neuroinflammation cytokines (interleukin-1b and tumor necrosis factor-α) were assessed in the prefrontal lobe of rat brains exposed to thimerosal in different timeframes. A total of 108 neonatal Wistar rats were divided into three groups having three subgroups. The experimental groups received a single dose of thimerosal (300 μg/kg) postnatally at 7, 9, 11, and 15 days. The vehicle groups received similar injections of phosphate-buffered saline in a similar manner. The control groups received nothing. Samples of the prefrontal cortex were collected and prepared for stereological, immunohistochemical, and molecular studies at timeframes of 12 or 48 h (acute phase) and 8 days (subchronic phase) after the last injection. The average density of the microglia and MCs increased significantly in the experimental groups. This increase was more evident in the 48 h group. At 8 days after the last injection, there was a significant decrease in the density of the MCs compared to the 12 and 48 h groups. Alterations in pro-inflammatory cytokines were significant for all timeframes. This increase was more evident in the 48 h group after the last injection. There was a significant decrease in both neuroinflammatory cytokines at 8 days after the last injection. It was found that ethylmercury caused abnormal neurogenic inflammatory reactions and alterations in the neuroimmune cells that remained for a longer period in the brain than in the blood. [ABSTRACT FROM AUTHOR]
- Subjects :
- *THIMEROSAL
*CYTOKINES
*HEAVY metal toxicology
*MICROGLIA
*MAST cells
Subjects
Details
- Language :
- English
- ISSN :
- 01480545
- Volume :
- 42
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Drug & Chemical Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 134969087
- Full Text :
- https://doi.org/10.1080/01480545.2018.1465949