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Genesis of the αβ T-cell receptor.

Authors :
Dupic, Thomas
Marcou, Quentin
Walczak, Aleksandra M.
Mora, Thierry
Source :
PLoS Computational Biology. 3/4/2019, Vol. 15 Issue 3, p1-19. 19p. 1 Diagram, 2 Charts, 4 Graphs.
Publication Year :
2019

Abstract

The T-cell (TCR) repertoire relies on the diversity of receptors composed of two chains, called α and β, to recognize pathogens. Using results of high throughput sequencing and computational chain-pairing experiments of human TCR repertoires, we quantitively characterize the αβ generation process. We estimate the probabilities of a rescue recombination of the β chain on the second chromosome upon failure or success on the first chromosome. Unlike β chains, α chains recombine simultaneously on both chromosomes, resulting in correlated statistics of the two genes which we predict using a mechanistic model. We find that ∼35% of cells express both α chains. Altogether, our statistical analysis gives a complete quantitative mechanistic picture that results in the observed correlations in the generative process. We learn that the probability to generate any TCRαβ is lower than 10−12 and estimate the generation diversity and sharing properties of the αβ TCR repertoire. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1553734X
Volume :
15
Issue :
3
Database :
Academic Search Index
Journal :
PLoS Computational Biology
Publication Type :
Academic Journal
Accession number :
135048521
Full Text :
https://doi.org/10.1371/journal.pcbi.1006874