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Elucidation of the structure of the core region and the complete structure of the R-type lipopolysaccharide of Erwinia carotovora FERM P-7576.
- Source :
-
European Journal of Biochemistry . 11/15/97, Vol. 250 Issue 1, p55-62. 8p. - Publication Year :
- 1997
-
Abstract
- An R-type lipopolysaccharide (LPS) from Erwinia carotovora strain FERM P-7576 was studied after strong alkaline degradation and mild acid hydrolysis. The resulting products were analyzed by fast-atom bombardment mass spectrometry, one- and two-dimensional 1H and 13C NMR spectroscopy, dephosphorylation and methylation analysis. The following structure was proposed for the core region of the LPS: [This symbol cannot be presented in ASCII format] where Hep is L-glycero-D-manno-heptose and Kdo is 3-deoxy-D-manno-octulosonic acid. Some LPS species lack the β-D GAlp residue or the β-D-Galp-(1→7)-α-Hepp disaccharide. With the known structures of lipid A [Fukuoka, S., Kamishima, H., Nagawa. Y., Nakanishi, H., Ishikawa, K., Niwa, Y., Tamiya, E. & Karube, I. (1992) Arch. Microbiol. 157, 311–318] and the core moiety, the complete LPS structure was established and confirmed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of the native and O-deacylated LPS. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 250
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13518096
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1997.00055.x