Cucumber mosaic virus coat protein: The potential target of 1, 4-pentadien-3-one derivatives.

Abstract Cucumber mosaic virus coat protein (CMV CP) plays a key role in cell-to-cell movement in host organisms. 1,4-Pentadien-3-one derivatives have excellent antiviral activities. In this study, we cloned, expressed and purified a CP recombinant protein. Then, we studied the binding interactions of CMV CP and 1, 4-pentadien-3-one derivatives N1–N20. Microscale thermophoresis experiments showed that N12 and N16 bound to CMV CP with dissociation constants of 0.071 and 0.11 μM, respectively. Docking and site-directed mutagenesis studies provided further insights into the interactions of N12 and N16 with Ile210, Thr69 and Ser213of CMV CP. Thus, these CMV CP residues may be important binding sites for the 1,4-pentadien-3-one derivatives N12 and N16. The data are important for designing and synthesizing new pentadienone derivatives. Graphical abstract Unlabelled Image Highlights • CMV CP proteins was overexpressed and identified as a target for antiviral compounds. • Compounds N12 and N16 directly interacts with CMV CP via hydrogen bonding with the Ile210, Thr69 and Ser213, respectively. • The Ile210, Thr69 and Ser213 of CMV CP are important for designing and synthesizing new pentadienone derivatives. [ABSTRACT FROM AUTHOR]