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HPAEC-PAD and Q-TOF-MS/MS analysis reveal a novel mode of action of endo-β-1,3(4)-d-glucanase Eng16A from coprinopsis cinerea on barley β-glucan.
- Source :
-
Food Chemistry . Jul2019, Vol. 287, p160-166. 7p. - Publication Year :
- 2019
-
Abstract
- Highlights • Q-TOF-MS/MS analysis reveals a novel mode of Eng16A action on barley β-glucan. • Eng16A can cleave (1 → 4)-β-linkages adjacent to two sides of the (1 → 3)-β-linkage. • Insertion of a (1 → 3)-β-linkage makes (1 → 4)-β-linkages prone to cleavage by Eng16A. Abstract We previously reported that an endo-β-1,3(4)- d -glucanase, Eng16A, from C. cinerea shows a higher degradation activity toward barley β-glucan than laminarin. HPAEC-PAD and Q-TOF-MS/MS analyses show that Eng16A-digestion products of barley β-glucan not only contain some oligosaccharides with (1 → 3)-β-linkage adjacent to the reducing end, which is consistent with β-1,3(4)-glucanase-digestion products, but also include some oligosaccharides containing (1 → 4)-β-linkage adjacent to the reducing end which is consistent with cellulase-digestion products. Thus, Eng16A possesses both cellulase and β-1,3(4)-glucanase activities. Because Eng16A does not degrade cellulose, we propose that the insertion of a (1 → 3)-β-linkage among the groups of (1 → 4)-β-linkages may make these (1 → 4)-β-linkages prone to cleavage by Eng16A. Furthermore, Eng16A also possesses transglycosylation activity which leads to some products containing one or a few consecutive (1 → 3)-β-linkages adjacent to the non-reducing end. Therefore, HPAEC-PAD and Q-TOF-MS/MS analyses provide an efficient approach to reveal complicated modes of action of some endo-β-1,3(4)- d -glucanases on barley β-glucan. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GLUCANS
*BETA-glucans
*BARLEY
Subjects
Details
- Language :
- English
- ISSN :
- 03088146
- Volume :
- 287
- Database :
- Academic Search Index
- Journal :
- Food Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 135198673
- Full Text :
- https://doi.org/10.1016/j.foodchem.2019.02.086