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Development of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatography.
- Source :
-
Journal of Medical Biochemistry . Apr2019, Vol. 38 Issue 2, p172-180. 9p. - Publication Year :
- 2019
-
Abstract
- To establish a fast and simple quantitative method for detection of heart-type fatty acid-binding protein (H-FABP) in serum based on a background fluorescence quenching immunochromatographic assay. A detection card based on the double-antibody sandwich double-antibody method with background fluorescence quenching was developed for quantitative measurement of H-FABP in serum. The optimal concentrations of control for coating the test and control lines were determined as well as the concentrations of gold-labeled antibodies used in preparing the detection system. The detection method for H-FABP in serum was established and validated using real-world clinical samples. The optimal concentrations of labeling antibody and coating antibody were 5.0 μg/mL and 1.0 mg/mL, respectively. The test card had a sensitivity of 1.15 ng/mL over a linear concentration range of 0–100 ng/mL. Based on three batches prepared for testing the card, the relative standard deviation (RSD) within batches was less than 15% without a significant difference (P=0.942). The detection method was tested against common interfering substances in serum, such as bilirubin, triglyceride and serum anticoagulants ethylenediamine tetraacetic acid (EDTA), heparin, and sodium citrate, and no significant cross-reaction was detected. The test method was further validated with 50 clinical serum samples, and the test results were comparable with standard reference detection methods with good correlation (R=0.95). Our study presents a new method with strong specificity and sensitivity for the detection of H-FABP in serum, which could promote H-FABP detection in a broad range of applications. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HEPARIN
*FATTY acid-binding proteins
*FLUORESCENCE quenching
Subjects
Details
- Language :
- English
- ISSN :
- 14528258
- Volume :
- 38
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Medical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 135355431
- Full Text :
- https://doi.org/10.2478/jomb-2018-0017