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Drosophila Trf4-1 involves in mRNA and primary miRNA transcription.
- Source :
-
Biochemical & Biophysical Research Communications . Apr2019, Vol. 511 Issue 4, p806-812. 7p. - Publication Year :
- 2019
-
Abstract
- Abstract Drosophila Trf4-1 (DmTrf4-1) is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4-1 resulted in the reduction of mRNAs and primary miRNAs (pri-miRNAs) in both Drosophila S2 cells and adult flies. Interestingly, the role of Trf4-1 in transcription is independent of its PAP/TENT activity. Moreover, using the chromatin immunoprecipitation assay, we uncovered that the loss of Trf4-1 led to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. Thus, our study indicates a positive role of Trf4-1 in the transcription of mRNAs and pri-miRNAs. Highlights • Drosophila Trf4-1 regulates the transcription of primary miRNAs as well as mRNAs. • The role of DmTrf4-1 in transcription is independent of its PAP/TENT activity. • Loss of DmTrf4-1 leads to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RNA polymerase II
*DROSOPHILA
*MICRORNA
*MESSENGER RNA
*GENETIC transcription
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 511
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 135491594
- Full Text :
- https://doi.org/10.1016/j.bbrc.2019.02.129