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Compound Phyllanthus urinaria L Inhibits HBV-Related HCC through HBx-SHH Pathway Axis Inactivation.
- Source :
-
Evidence-based Complementary & Alternative Medicine (eCAM) . 3/24/2019, p1-15. 15p. 1 Diagram, 1 Chart, 9 Graphs. - Publication Year :
- 2019
-
Abstract
- Compound Phyllanthus urinaria L (CP) is a traditional formula widely used in clinical practice for hepatocellular carcinoma (HCC), especially HBV-related HCC. HBx, HBV X gene encoded X protein, has positive correlation with the abnormal SHH pathway in HBV-related HCC. So, we predicted that CP has the capability of anti-HBV-related HCC maybe via inactivating the HBx-Hedgehog pathway axis. HepG2-HBx cells, HBx overexpression, were treated with CP (70μg/ml and 35 μg/ml, respectively) for 48 hours and the mice which received the HepG2-HBx cells were treated with CP (625mg/kg and 300 mg/kg, respectively) for 17 days to evaluate the effect of CP on HBV-related HCC. HBx could accelerate HepG2 cells proliferation, clone formation, and migration in vitro and also could strengthen tumor growth in mice. However, CP could significantly decrease HepG2-HBx cells proliferation, clone formation, and migration in vitro and also could inhibit tumors growth in mice in a dose-dependent manner. Mechanism studies suggested that HBx upregulated the mRNA and proteins expression of Sonic hedgehog (SHH), transmembrane receptor patched (PTCH-1), smoothened (SMO), oncogene homolog transcription factors-1 (GLI-1), and oncogene homolog transcription factors-2 (GLI-2), which are compositions of the SHH pathway. CP could inhibit the mRNA and proteins expression of SHH, PTCH-1, GLI-1, and HBx. It may be one of the underlying mechanisms of CP to delay the HBV-related HCC development through the HBx-SHH pathway axis inactivation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEIN metabolism
*CELL proliferation
*ANIMAL experimentation
*ANTINEOPLASTIC agents
*CELL motility
*CELLULAR signal transduction
*DOSE-effect relationship in pharmacology
*GENE expression
*GENES
*GENETIC techniques
*HEPATITIS B
*HEPATOCELLULAR carcinoma
*MESSENGER RNA
*MICE
*MOLECULAR structure
*TRANSCRIPTION factors
*PLANT extracts
*IN vitro studies
*TUMOR grading
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Academic Search Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 135508328
- Full Text :
- https://doi.org/10.1155/2019/1635837