Back to Search Start Over

A preliminary attempt to explore the potential functions of a tetraspanin gene (MmTSPAN) in the innate immunity of hard clam Meretrix meretrix: Sequence features and expression profiles.

Authors :
Wang, Yan
Wang, Mengqiang
Wang, Baojie
Liu, Mei
Jiang, Keyong
Hou, Xuguang
Wang, Lei
Source :
Fish & Shellfish Immunology. May2019, Vol. 88, p135-141. 7p.
Publication Year :
2019

Abstract

Abstract Tetraspanins belong to the transmembrane 4 superfamily (TM4SF), and play crucial roles in immune responses. In the present study, a novel tetraspanin gene (designated MmTSPAN) was cloned and characterized from the hard clam Meretrix meretrix. The complete cDNA sequence of MmTSPAN contained an open reading frame (ORF) of 816 bp, which encoded a protein of 271 amino acids. Mm TSPAN exhibited highly similarity with previously identified tetraspanins from other species. It contained four transmembrane domains (12–35 aa, 69–92 aa, 99–123 aa and 238–261 aa), characteristic CCG motif and four conservative cysteine residues. The mRNA transcripts of MmTSPAN were ubiquitously detectable in all the tested tissues, with the highest expression level in hepatopancreas. Temporal transcriptional levels in the hepatopancreas revealed significant up-regulation of MmTSPAN by Vibrio splendidus stimulation, with a 3.14-fold increase at 6 h compared to the control, and reaching 32.98-fold at 24 h. These results provide useful information for further study of the function of tetraspanin in the innate immune system of M. meretrix , and may offer a new therapeutic target for diseases of M. meretrix. Highlights • A novel tetraspanin gene (MmTSPAN) was identified and characterized from Meretrix meretrix. a. • MmTSPAN was highly expressed in hepatopancreas and gill. • Significant induction of MmTSPAN was detected post Vibrio splendidus stimulation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10504648
Volume :
88
Database :
Academic Search Index
Journal :
Fish & Shellfish Immunology
Publication Type :
Academic Journal
Accession number :
135625557
Full Text :
https://doi.org/10.1016/j.fsi.2019.01.048