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Ester flavorants detection in foods with a bienzymatic biosensor based on a stable Prussian blue-copper electrodeposited on carbon paper electrode.
- Source :
-
Talanta . Jul2019, Vol. 199, p541-546. 6p. - Publication Year :
- 2019
-
Abstract
- Abstract A stable and reproducible layer of Prussian blue (PB) modified with copper was electrodeposited on carbon paper electrodes for the multiple detection of ester flavorants with a bienzymatic biosensor. Carbon fiber composite paper was investigated as high-surface, low-cost substrate for biosensor development. The pre-activation of the electrode surface by cyclic voltammetry was necessary to improve the electrochemical properties before the electrochemical deposition of Prussian blue-copper film (PB-Cu). The stability and the reproducibility of the obtained PB-Cu carbon paper electrode was demonstrated at pH 7.4, optimum for biosensor development. The developed biosensor is based on the immobilization of two enzymes (carboxyl esterase and alcohol oxidase) by cross-linking with glutaraldehyde onto PB-Cu carbon paper electrode. A mixture of key aroma ester compounds (methyl butyrate, ethyl butyrate, methyl cinnamate and ethyl cinnamate) was detected in several food samples with low interferences. Graphical abstract fx1 Highlights • Prussian blue (PB) modified with copper with improved stability at neutral pH. • Carbon fiber composite paper is a high-surface, low-cost substrates excellent for biosensor manufacturing. • Total content of ester flavorants mixtures in foods detection. • Bienzymatic biosensor based on carboxyl esterase and alcohol oxidase. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 199
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 135709739
- Full Text :
- https://doi.org/10.1016/j.talanta.2019.02.094