شناسایی ژنهای بتالاکتاماز در اشرشیاکلی جداشده از زخم پای دیابتی به روش Multiplex PCR

Introduction: Diabetic foot ulcer is a complication of diabetes mellitus and can be associated with an increased risk of amputation or even death as a result of a wound infection. E.coli can lead to worsening of the condition of the wound. The aim of this study was to identify β-lactamase genes in E. coli isolated from diabetic foot ulcers Materials and Methods: In this experimental study, 110 isolates of E. coli from patients with diabetic foot ulcers hospitalized in the infectious ward of the in Peymanieh, Jahrom and Ali Asghar and Namazi, Shiraz, hospitals in 1396 were selected. Determination of antibiotic susceptibility, phenotypic identification of strains producing broad-spectrum beta-lactamases (ESBLs), strains producing MBL and strains producing Carbapenemas was done. The existence of genes encoding bla TEM, bla SHV and bla OXA-1 enzymes was investigated by multiplex PCR assay Results: In this study, 35 isolates of Escherichia coli were isolated. 13 isolates produced broad-spectrum betalactamases. Genetically, 19 isolates with TEM gene (54.2%) and 3 isolates with SHV (8.5%), which simultaneously had 1 isolate from 3 isolates with SHV, also had OXA-1 gene (2.8%). The highest susceptibility of Escherichia coli was to maropenem (99%) and the lowest antibiotic susceptibility to amikacin (3.2%). Conclusion: According to the results of this study and monitoring of antibiotic resistance in patients with diabetic foot ulcers, treatment of infections caused by this agent can be facilitated. [ABSTRACT FROM AUTHOR]