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The Kinetics of the Anaerobic Reduction of Fungal Laccase B.
- Source :
-
European Journal of Biochemistry . 1973, Vol. 34 Issue 3, p434-439. 6p. - Publication Year :
- 1973
-
Abstract
- 1. A stopped-flow technique was used to study the anaerobic reduction of fungal laccase B by hydroquinone, ascorbate and Fe(CN)4-6. The reactions were followed by measurements of the intensity of absorption bands at 610 and 330 nm, related to a one- and a two-electron acceptor, respectively. 2. The experiments confirmed earlier results indicating a second-order reduction of the 610-nm chromophore. The reduction of the 330-nm chromophore was found to be constant with the concept that the rate is limited by an intramolecular step. A model for the anaerobic reduction of laccase was formulated. 3. The effect of F- on the anaerobic kinetics implies that this inhibitory anion changes the rate constant of the rate-limiting step. 4. The reduction of the 330-nm chromophore, as it appears under anaerobic conditions, cannot be part of the normal catalytic mechanism. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LACCASE
*ANAEROBIC bacteria
*FUNGI
*ELECTRONS
*HYDROQUINONE
*ANIONS
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 34
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13602351
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1973.tb02776.x