Fluorescent detection of ascorbic acid using glutathione stabilized Au nanoclusters.

The fluorescence of Au nanoclusters can be quenched at the presence of ferrous and hydrogen peroxide and recovered as ascorbic acid added. • Au Nanoclusters is synthetized through chemical reduction. • It can be used for qualitative analysis of ascorbic acid. • The detection limit is 200 μM and linear range is 350–700 μM. Au nanoclusters were synthetized through chemical reduction based on glutathione template. The fluorescence excitation and emission wavelength is 375 nm and 565 nm, respectively. The fluorescence would be quenched at the presence of ferrous and hydrogen peroxide and recovered as ascorbic acid added. It can be used for qualitative analysis of ascorbic acid according to fluorescence recovery. The fluorescence intensity is positive correlation with the concentration of ascorbic acid. The detection limit is 200 μM and linear range is 350–700 μM. It offers an easy and convenient method to detect of ascorbic acid. [ABSTRACT FROM AUTHOR]