Anammox microbial community and activity changes in response to water and dissolved oxygen managements in a paddy-wheat soil of Southern China.

Anammox are unusual members of the microbial community contributing to N losses via anaerobic ammonium oxidation. Anammox use nitrite as a substrate and produce hydrazine as an intermediate product. Up to date, the effects of dissolved oxygen and moisture dynamics on anammox potential and microbial community in agricultural soils were poorly understood. In this study, we investigated the interaction of dissolved oxygen and moisture contents as factors affecting the soil anammox process. The experiment had four fertilization treatments i.e. Control (CK), Chemical fertilizer (CF), Pig composted manure plus chemical fertilizer (PMCF), and Straw returned to soil plus chemical fertilizer (SRCF) with different water contents, 70%-FC, Alternate wetting and drying (AWD), Flooding I (D.O 5.8 mg L−1), and Flooding II (D.O 2.6 mg L−1). 15N-isotopic tracer technique was used to evaluate the anammox and denitrification rates. The results showed that the anammox rate ranged from the lowest 0.56 nmol N 2 ·g−1·h−1 in CF (with 70% FC water) to the highest rate of 1.47 nmol N 2 ·g−1·h−1 in SRCF (with flooding II). In water treatments, the average lowest and highest anammox rates were in the 70% FC (0.61 nmol N 2 ·g−1·h−1) and Flooding II (1.14 nmol N 2 ·g−1·h−1), respectively. Moreover, under soil treatments, the minimum average anammox rate was found in the PMCF (0.76 nmol N 2 ·g−1·h−1) and maximum in the SRCF (1.01 nmol N 2 ·g−1·h−1). Interestingly, anammox genes copy numbers were highest in alternate wetting and drying conditions under all fertilizer treatments rather than in continuous flooding. The phylogenetic analysis showed that Ca. Brocadia was dominating while some of Ca. Jettenia was also present. In conclusion, alternate wetting and drying could increase the number of anammox bacteria and microbial diversity. Unlabelled Image • Alternate wetting and drying (AWD) can enhance Anammox rate and microbial diversity. • Consistently higher dissolved oxygen can inhibit the Anammox process/activity. • Long-term application of pig manure can decrease the Anammox activity. • Anammox activity has a positive relationship with hydrazine gene abundance. [ABSTRACT FROM AUTHOR]