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Amphidinolide H, a novel type of actin-stabilizing agent isolated from dinoflagellate
- Source :
-
Biochemical & Biophysical Research Communications . Jul2004, Vol. 320 Issue 3, p961-965. 5p. - Publication Year :
- 2004
-
Abstract
- The effect of novel cytotoxic marine macrolide, amphidinolide H (Amp-H), on actin dynamics was investigated in vitro. Amp-H attenuated actin depolymerization induced by diluting F-actin. This effect remained after washing out of unbound Amp-H by filtration. In the presence of either Amp-H or phalloidin, lag phase, which is the rate-limiting step of actin polymerization, was shortened. Phalloidin decreased the polymerization-rate whereas Amp-H did not. Meanwhile, the effects of both compounds were the same when barbed end of actin was capped by cytochalasin D. Quartz crystal microbalance system revealed interaction of Amp-H with G-actin and F-actin. Amp-H also enhanced the binding of phalloidin to F-actin. We concluded that Amp-H stabilizes actin in a different manner from that of phalloidin and serves as a novel pharmacological tool for analyzing actin-mediated cell function. [Copyright &y& Elsevier]
- Subjects :
- *DINOFLAGELLATES
*ACTIN
*PHALLOIDINE
*CYTOCHALASINS
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 320
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 13625856
- Full Text :
- https://doi.org/10.1016/j.bbrc.2004.06.050