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Loop-mediated isothermal amplification assays for Enterococcussp., Escherichiacoli and Staphylococcusaureus in chicken.

Authors :
Song, HyeSoon
Bae, YouChan
Kwon, HyukMan
Kwon, YongKuk
Joh, SeongJoon
Source :
FEMS Microbiology Letters. Mar2019, Vol. 366 Issue 5, pN.PAG-N.PAG. 1p. 5 Diagrams, 2 Charts.
Publication Year :
2019

Abstract

Bacterial chondronecrosis with osteomyelitis (BCO) is a major cause of lameness in broiler chicken, and results in serious economic losses worldwide. Although the pathogenesis mechanism leading to lameness is not entirely understood, some strains of Enterococcus sp. avian pathogenic Escherichia coli or Staphylococcus aureus have been long recognized as important causative pathogens. To prevent the progression of Enterococcus sp. avian pathogenic E. coli or S. aureus infections, we developed rapid, sensitive and convenient diagnostic assays using loop-mediated isothermal amplification (LAMP). Entero-Common-LAMP assays were developed for simultaneous detection of eight Enterococcus species. To target specific microorganisms, seven Entero-Specific-LAMP assays for E. faecalis, E. faecium, E. hirae, E. gallinarum, E. avium, E. durans and E. cecorum were developed, as well as E. coli -LAMP and S. aureus -LAMP assays. Considering the prevalence and economic impact of Enterococcus sp. E. coli and S. aureus, the 10 different LAMP assays which were developed have considerable potential as routine diagnostic methods in the field or in resource-limited environments. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03781097
Volume :
366
Issue :
5
Database :
Academic Search Index
Journal :
FEMS Microbiology Letters
Publication Type :
Academic Journal
Accession number :
136278952
Full Text :
https://doi.org/10.1093/femsle/fnz042