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Modifying Graft-versus-Host Disease in a Humanized Mouse Model by Targeting Macrophages or B-Cells.
- Source :
-
Journal of Immunology Research . 5/8/2019, p1-14. 14p. 4 Charts, 5 Graphs. - Publication Year :
- 2019
-
Abstract
- Humanized mouse models can well be modified to study specific aspects of Graft-versus-Host Disease (GvHD). This paper shows the results of both macrophage depletion and (early) B-cell depletion in a humanized mouse model using RAG2-/-γc-/- mice injected with HuPBMCs. Macrophage depletion showed a significant decrease in survival and also lead to a change in the histomorphology of the xenogeneic reaction. Higher levels of infiltrating B-cells were observed in various organs of mice depleted for macrophages. With (early) B-cell depletion using Rituximab, a clear improvement on clinical symptoms was observed, even when probably only inactivated B-cells were deleted. However, the histological examinations only showed a significant morphological effect on liver fibrosis. This may be related to a difference in the mRNA levels of TGF-β. Also, lower mRNA levels of Tregs in some organs were observed after Rituximab treatment, which contradicts that a higher number of Tregs would always be related to less severe GvHD. Our data show that both macrophage depletion and (early) B-cell depletion in a xenogeneic mouse model can influence the clinical, histological, and cytokine production of a GvHD response. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GRAFT versus host disease
*B cells
*MACROPHAGES
*MOUSE diseases
*THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 23148861
- Database :
- Academic Search Index
- Journal :
- Journal of Immunology Research
- Publication Type :
- Academic Journal
- Accession number :
- 136284308
- Full Text :
- https://doi.org/10.1155/2019/3538963