分泌性中耳炎患者外周血Th1Th2 细胞及T淋巴细胞亚群水平的 表达及临床意义.

Objective: To investigate the expression and clinical significance of T helper cell 1(Th1), T helper cell 2(Th2)cytokines and T lymphocyte subsets in peripheral blood of patients with secretory otitis media(SOM). Methods: 135 patients with SOM who were admitted to our hospital from January 2015 to January 2018 were selected as SOM group. According to the course of disease,the patients were divided into acute group(the duration<14 d, 49 cases), subacute group(the duration of 14-30 d, 53 cases) and chronic group(the duration>30 d, 33 cases). 100 healthy patients who had physical examination in the outpatient department of our hospital were selected as the control group. The levels of peripheral blood Th1 cytokines [interferon(INF-γ), interleukin-2(IL-2)], Th2 cytokines[interleukin-4(IL-4), interleukin-10(IL-10)] and T lymphocyte subsets [CD3+, CD4+, CD8+, CD4+/CD8+] were compared between SOM group and control group, and patients with different course of SOM. Pearson correlation analysis was performed on the correlation between IFN-γ, IL-2, IL-4, IL-10 and CD3+, CD4+, CD8+, CD4+/CD8+. Results: The levels of peripheral blood IFN-γ, IL-2, IL-4 and IL-10 in the SOM patients were higher than those of the control group(P<0.05). The levels of peripheral blood INF-γ, IL-2, IL-4 and IL-10 in the acute group were lower than those of the subacute group and the chronic group. The levels of peripheral blood INF-γ, IL-2,IL-4 and IL-10 in the subacute group were lower than those of chronic group(P<0.05). The levels of peripheral blood CD3+, CD4+and CD4+/CD8+in the SOM patients were lower than those in the control group, and the levels of CD8+were higher than those in the control group(P<0.05). The level of CD3+, CD4+and CD4+/CD8+in the acute group were higher than those in the subacute group and chronic group. The level of CD8+in the acute group were lower than that in the subacute group and chronic group. The levels of CD3+, CD4+and CD4+/CD8+in the subacute group were higher than those in the chronic group. The level of CD8+was lower than that of chronic group(P<0.05).Pearson correlation analysis showed that peripheral blood INF-γ and IL-4 were positively correlated with CD8+in the SOM patients(P<0.05), while IL-4 was negatively correlated with CD3+, CD4+(P<0.05). Conclusion: The levels of peripheral blood Th1 Th2 cytokines and T lymphocyte subsets in the SOM patients are abnormal, and their levels are associated with the occurrence and development of diseases.The monitoring of Th1 Th2 cytokines and T lymphocyte subsets helps to assess the condition of SOM patients. [ABSTRACT FROM AUTHOR]