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Antifungal activity of analogues of antimicrobial peptides isolated from bee venoms against vulvovaginal Candida spp.
- Source :
-
FEMS Yeast Research . May2019, Vol. 19 Issue 3, pN.PAG-N.PAG. 1p. 4 Diagrams, 3 Charts, 2 Graphs. - Publication Year :
- 2019
-
Abstract
- Candida albicans is the main causative agent of vulvovaginal candidiasis (VVC), a common mycosis in women, relapses of which are difficult to manage due to biofilm formation. This study aimed at developing novel non-toxic compounds active against Candida spp. biofilms. We synthesised analogues of natural antifungal peptides LL-III (LL-III/43) and HAL-2 (peptide VIII) originally isolated from bee venoms and elucidated their structures by nuclear magnetic resonance spectroscopy. The haemolytic, cytotoxic, antifungal and anti-biofilm activities of LL-III/43 and peptide VIII were then tested. LL-III/43 and VIII showed moderate cytotoxicity to HUVEC-2 cells and had comparable inhibitory activity against C. albicans and non- albicans spp. The lowest minimum inhibitory concentration (MIC90) of LL-III/43 was observed towards Candida tropicalis (0.8 µM). That was 8-fold lower than that of antimycotic amphotericin B. Both peptides can be used to inhibit Candida spp. bio film f ormation. Biofilm inhibitory concentrations (BIC50) ranged from 0.9 to 58.6 µM and biofilm eradication concentrations (BEC50) for almost all tested Candida spp. strains ranged from 12.8 to 200 µM. Als o pro ven were the peptides' abilities to reduce the area colonised by biofilms, inhibit hyphae formation and permeabilise cell membranes in biofil ms. LL-III/43 and VIII are promising candidates for further development as therapeutics against VVC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15671356
- Volume :
- 19
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- FEMS Yeast Research
- Publication Type :
- Academic Journal
- Accession number :
- 136465816
- Full Text :
- https://doi.org/10.1093/femsyr/foz013