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Detection of casein kinase II by aggregation-induced emission.
- Source :
-
Talanta . Aug2019, Vol. 201, p450-454. 5p. - Publication Year :
- 2019
-
Abstract
- A novel aggregation-induced emission (AIE) probe comprised of a hydrophilic protein kinase specific peptide and a hydrophobic tetraphenylethene (TPE) unit was synthesized through click reaction. The prepared TPE-peptide probe could be completely degraded by carboxypeptidase Y (CPY) to release hydrophobic TPE part, which aggregated in buffer solution and showed strong TPE emission. In the presence of casein kinase (CKII), the phosphorylation of peptide prevented the complete degradation by CPY producing the nonemissive probe. Thus, the developed probe can be used to detect CKII homogeneously and conveniently. This detection process can be finished within 1.5 h with high sensitivity (0.05 mU/μL) and good selectivity. The developed method can also be used to screen protein kinase inhibitor even in a complex biological system. Image 1 • A novel AIE probe was synthesized through click reaction. • This probe can be used to detect CKII homogeneously within 1.5 h. • This method can be used to screen protein kinase inhibitor even in a complex biological system. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 201
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 136540605
- Full Text :
- https://doi.org/10.1016/j.talanta.2019.04.036