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A highly sensitive fluorescent probe for hydrogen sulfide based on dicyanoisophorone and its imaging in living cells.
- Source :
-
Sensors & Actuators B: Chemical . Sep2019, Vol. 294, p141-147. 7p. - Publication Year :
- 2019
-
Abstract
- • A dicyanoisophorone-based fluorescent probe was developed for sensing H 2 S with a large Stokes shift (155 nm). • The probe showed faster time response and high sensitivity for H 2 S. • The probe can be used to detect exdogenous and endogenous H 2 S in living HeLa cells. Due to the importance of hydrogen sulfide (H 2 S) in physiology and pathology, the development of fluorescence probes for the detection of H 2 S has attracted extensive attention of researchers. We developed a H 2 S fluorescence probe (DCN-S) based on dicyanoisopentanone using dithioether and ester groups as the reaction sites. Under the nucleophilic substitution of H 2 S, disulfide bond of DCN-S was broken. Then the probe changed into dicyanoisophorone derivative and emitted an orange fluorescence band from 530 nm to 700 nm (centred 580 nm). Upon response for H 2 S, DCN-S exhibited high sensitivity (88 nM detection limit) and large stokes shift (155 nm). Importantly, the probe also was successfully used for endogenous and exogenous imaging of living cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 294
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 136690745
- Full Text :
- https://doi.org/10.1016/j.snb.2019.05.038