Back to Search
Start Over
A highly specific and ultrasensitive probe for the imaging of inflammation-induced endogenous hypochlorous acid.
- Source :
-
Analyst . 6/7/2019, Vol. 144 Issue 11, p3546-3551. 6p. - Publication Year :
- 2019
-
Abstract
- A turn-on two-photon fluorescent probe HCA-Green for hypochlorous acid (HOCl) was synthesized using 4-methylamino-1,8-naphthalimide (MNA) as a two-photon fluorophore and p-hydroxyaniline as a leaving-recognition domain. Both the probe and the fluorophore were investigated under one- and two-photon excitation modes. The fluorescence intensity of the probe was enhanced by ∼229-fold and ∼193-fold under one-photon and two-photon excitation, respectively, after reacting with HOCl. A maximal two-photon action cross-section of 50 GM was obtained under excitation at 810 nm. The probe exhibited high sensitivity with a detection limit of 42.3 nM, as well as high selectivity, low cytotoxicity, and good photostability. Two-photon microscopy (TPM) was conducted to visualize HOCl levels in living cells and tissues. The production of endogenous HOCl induced by lipopolysaccharide-mediated inflammation was successfully monitored with this probe. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HYPOCHLORITES
*FLUORESCENT probes
*DETECTION limit
*LIPOXINS
*FLUORESCENCE
Subjects
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 144
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 136691746
- Full Text :
- https://doi.org/10.1039/c9an00390h