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Influence of post‐thawing thermal environment on bovine sperm characteristics and in vitro fertility.
- Source :
-
Andrologia . Jul2019, Vol. 51 Issue 6, pN.PAG-N.PAG. 1p. - Publication Year :
- 2019
-
Abstract
- Our aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer‐Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment ≥36.0°C for 4 hr. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SPERMATOZOA
*FERTILITY
*ECOLOGY
*CELL motility
*THERMAL stresses
Subjects
Details
- Language :
- English
- ISSN :
- 03034569
- Volume :
- 51
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Andrologia
- Publication Type :
- Academic Journal
- Accession number :
- 136767157
- Full Text :
- https://doi.org/10.1111/and.13266